Exploring Bikaverin as Metal ion Biosensor: A Computational approach

Abstract

A computational exploration of fungi produced pigment bikaverin as a biosensor towards bioavailable metal ions is presented. Systematic studies of the optimized ground and excited state geometries were attempted for exploring metal ion binding pocket, comparative binding propensity and optical properties of the bikaverin and its adducts with studied metal ions. The screening of thirteen (13) bioavailable metal ions, revealed a range of binding strengthtowards bikaverin receptor with Ca²⁺, Mg²⁺ and Al³⁺as the strongest binders. Besides, upon binding to bikaverin receptor an enhancement in its fluorescence intensity was observed in the order Ca²⁺> Al³⁺> Mg²⁺. The computationally predicted selectivity of bikaverin receptor towards Ca²⁺was experimentally corroborated through the preliminary fluorescence studies. The bikaverin probe showed an enhancement of fluorescence emission in presence of Ca²⁺ ions in buffered aqueous medium.