HPLC-DAD analysis, antioxidant and antibacterial properties of fruit extracts from Pistacia atlantica Desf.

Younes Douffa; Karima Saffidine; Nour Elhouda Belabes; Nadjet Azzi; Haifaa Laroui; Hafsa Silini Cherif; Thoraya Guemmaz; Fatima Zerargui; Abderahmane Baghiani

doi:10.17344/acsi.2025.9317

Authors

Younes Douffa Laboratory of Applied Biochemistry, Faculty of Nature and Life sciences, University Ferhat Abbas Setif1, Setif 19000, Algeria https://orcid.org/0009-0000-6816-3808
Karima Saffidine Laboratory of Applied Biochemistry, Faculty of Nature and Life sciences, University Ferhat Abbas Setif1, Setif 19000, Algeria https://orcid.org/0000-0001-7122-961X
Nour Elhouda Belabes Laboratory of Applied Biochemistry, Faculty of Nature and Life sciences, University Ferhat Abbas Setif1, Setif 19000, Algeria https://orcid.org/0009-0007-3487-8291
Nadjet Azzi Laboratory of Applied Biochemistry, Faculty of Nature and Life sciences, University Ferhat Abbas Setif1, Setif 19000, Algeria https://orcid.org/0009-0004-9450-6594
Haifaa Laroui Laboratory of Applied Biochemistry, Faculty of Nature and Life sciences, University Ferhat Abbas Setif1, Setif 19000, Algeria https://orcid.org/0009-0008-4221-3924
Hafsa Silini Cherif Laboratory of Applied Microbiology, Department of Microbiology, Faculty of Natural and Life, Ferhat Abbas Setif1 University, Setif 19000, Algeria https://orcid.org/0000-0001-8609-5028
Thoraya Guemmaz Laboratory of Applied Biochemistry, Faculty of Nature and Life sciences, University Ferhat Abbas Setif1, Setif 19000, Algeria https://orcid.org/0000-0002-5074-3991
Fatima Zerargui Laboratory of Applied Biochemistry, Faculty of Nature and Life sciences, University Ferhat Abbas Setif1, Setif 19000, Algeria https://orcid.org/0000-0002-4059-3600
Abderahmane Baghiani Laboratory of Applied Biochemistry, Faculty of Nature and Life sciences, University Ferhat Abbas Setif1, Setif 19000, Algeria https://orcid.org/0000-0002-6881-011X

DOI:

https://doi.org/10.17344/acsi.2025.9317

Abstract

Pistacia atlantica is commonly used in traditional medicine to treat various diseases in Algeria. This study was carried out to investigate the antioxidant potential and antibacterial properties of fruit extracts. The results indicated various amounts of polyphenols and flavonoids in different extracts. Quercetin, gallic acid, chlorogenic acid and methyl gallate were the dominant constituents in the ethyl acetate extract (EAE) and crude extract (CrE) quantified by HPLC-DAD. EAE was the most active in scavenging DPPH and hydroxyl (OH^.) radicals, hydrogen peroxide (H₂O₂), reducing power and total antioxidant capacity. All extracts have the ability to inhibit lipid peroxidation. A broad spectrum of antibacterial effects (10.66 to 29.33 mm) was obtained. In addition, the time-kill assay and the MBC/MIC ratio indicated that all extracts were bactericidal against most of the test bacteria and their combination with antibiotics showed remarkable synergistic effect. The findings of this study suggest that medicinal plant is a potential source of natural antioxidant and antibacterial compounds, which could be used where these kind of activities are warranted.

HPLC-DAD analysis, antioxidant and antibacterial properties of fruit extracts from Pistacia atlantica Desf.

Authors

DOI:

Abstract

Downloads

Published

Issue

Section

License