Optimization of ultrasound-assisted extraction of phenols from seeds of grape pomace

Authors

  • Marko Zoran Anđelković University of Niš, Faculty of Natural Sciences and Mathematics, Department of Chemistry, Višegradska 33, 18000 Niš, Serbia
  • Ana Slobodan Milenković-Andjelković University of Niš, Faculty of Natural Sciences and Mathematics, Department of Chemistry, Višegradska 33, 18000 Niš, Serbia
  • Blaga Radovanović University of Niš, Faculty of Natural Sciences and Mathematics, Department of Chemistry, Višegradska 33, 18000 Niš, Serbia
  • Aleksandra Radovanović University of Belgrade, Faculty of Chemistry, Studentski trg 12-16, 11 000 Belgrade, Serbia

Keywords:

grape pomace seeds, ultrasound assisted extraction, phenolic compounds, radical scavenging activity, response surface methodology

Abstract

The aim of this research was to optimize extraction condition of ultrasound-assisted extraction (UAE) of phenols from red grape Vranac variety (Vitis vinifera L.) pomace seeds. The minimum experiments needed as adequate responses for optimization of UAE by response surface methodology (RSM) were obtained by spectrophotometric and HPLC analyses of seed extracts. UAE greatly depends on three independent variables: extraction temperature, time and liquid/solid ratio. The RSM can be used for optimization of UAE conditions to obtain maximum responses such as extraction yield, TPC, (+)-catechin, (-)-epicatechin and proanthocyanidin content. The predicted values of model are in accordance with experimental data under same conditions. Experimental data also confirmed that UAE gives better yield of phenolics than conventional solvent extraction. The UAE under optimal extraction conditions is suitable for obtaining extracts that are rich in phenolic content and strong antioxidant activity which could be used as additives in food and medicaments.

Author Biographies

Marko Zoran Anđelković, University of Niš, Faculty of Natural Sciences and Mathematics, Department of Chemistry, Višegradska 33, 18000 Niš, Serbia

University of Niš, Faculty of Natural Sciences and Mathematics, Department of Chemistry

Ana Slobodan Milenković-Andjelković, University of Niš, Faculty of Natural Sciences and Mathematics, Department of Chemistry, Višegradska 33, 18000 Niš, Serbia

University of Niš, Faculty of Natural Sciences and Mathematics, Department of Chemistry

Blaga Radovanović, University of Niš, Faculty of Natural Sciences and Mathematics, Department of Chemistry, Višegradska 33, 18000 Niš, Serbia

University of Niš, Faculty of Natural Sciences and Mathematics, Department of Chemistry, professor

Aleksandra Radovanović, University of Belgrade, Faculty of Chemistry, Studentski trg 12-16, 11 000 Belgrade, Serbia

University of Belgrade, Faculty of Chemistry

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Published

17.10.2014

Issue

Vol. 61 No. 4 (2014)

Section

Chemical, biochemical and environmental engineering

License

Except where otherwise noted, articles in this journal are published under the Creative Commons Attribution 4.0 International License