Artificial Neural Networks and Response Surface Methodology approach for optimization of an eco-friendly and detergent-stable lipase production from Actinomadura keratinilytica strain Cpt29

Authors

  • Noura Semache Laboratory of Applied Organic Chemistry, Chemistry Department, Faculty of Sciences, Badji Mokhtar University, P.O. Box 12, 23000 Annaba, Algeria
  • Fatiha Benamia Laboratory of Applied Organic Chemistry, Chemistry Department, Faculty of Sciences, Badji Mokhtar University, P.O. Box 12, 23000 Annaba, Algeria
  • Bilal Kerouaz Laboratory of Applied Biochemistry and Microbiology, Biochemistry Department, Faculty of Sciences, Badji Mokhtar University, P.O. Box 12, 23000, Annaba, Algeria
  • Inès Belhaj Laboratory of Biotechnology of Molecular Eukaryotes, Centre of Biotechnology of Sfax, University of Sfax, Road of Sidi Mansour Km 6, P.O. Box 1177, Sfax 3018, Tunisia
  • Selma Bounour Laboratory of Applied Organic Chemistry, Chemistry Department, Faculty of Sciences, Badji Mokhtar University, P.O. Box 12, 23000 Annaba, Algeria
  • Hafedh Belghith Laboratory of Biotechnology of Molecular Eukaryotes, Centre of Biotechnology of Sfax, University of Sfax, Road of Sidi Mansour Km 6, P.O. Box 1177, Sfax 3018, Tunisia
  • Ali Gargouri Laboratory of Biotechnology of Molecular Eukaryotes, Centre of Biotechnology of Sfax, University of Sfax, Road of Sidi Mansour Km 6, P.O. Box 1177, Sfax 3018, Tunisia
  • Ali Ladjama Laboratory of Applied Biochemistry and Microbiology, Biochemistry Department, Faculty of Sciences, Badji Mokhtar University, P.O. Box 12, 23000, Annaba, Algeria
  • Zeineddine Djeghaba Laboratory of Applied Organic Chemistry, Chemistry Department, Faculty of Sciences, Badji Mokhtar University, P.O. Box 12, 23000 Annaba, Algeria

DOI:

https://doi.org/10.17344/acsi.2020.6401

Keywords:

Lipase, Actinomadura keratinilytica, Optimization, RSM, ANN, Detergent.

Abstract

This work mainly focused on the production of an efficient, economical, and eco-friendly lipase (AKL29) from Actinomadura keratinilytica strain Cpt29 isolated from poultry compost in north east of Algeria, for use in detergent industries. AKL29 shows a significant lipase activity (45 U/mL) towards hydrolyzed triacylglycerols, indicating that it is a true lipase. For maximum lipase production the modeling and optimization of potential culture parameters such as incubation temperature, cultivation time, and Tween 80 (v/v) were built using RSM and ANN approaches. The results show that both the two models provided good quality predictions, yet the ANN showed a clear superiority over RSM for both data fitting and estimation capabilities. A 4.1-fold increase in lipase production was recorded under the following optimal condition: incubation temperature (37.9 °C), cultivation time (111 h), and Tween 80 (3.27%, v/v). Furthermore, the partially purified lipase showed good stability, high compatibility, and significant wash performance with various commercial laundry detergents, making this novel lipase a promising potential candidate for detergent industries.

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Published

15.09.2021

Issue

Vol. 68 No. 3 (2021)

Section

Chemical, biochemical and environmental engineering

License

Except where otherwise noted, articles in this journal are published under the Creative Commons Attribution 4.0 International License