Ionic Liquid-Based Dispersive Liquid–Liquid Microextraction for the Simultaneous Determination of Carbamazepine and Lamotrigine in Biological Samples

Abstract

This paper describes a new approach for the determination of carbamazepine and lamotrigine in biological samples by ionic liquid dispersive liquid-phase microextraction prior to high-performance liquid chromatography with ultraviolet detection. The effects of different ionic liquids (ILs) on the extraction efficiency of carbamazepine and lamotrigine were investigated. The highest extraction efficiencies of carbamazepine and lamotrigine were obtained using 30 µL of 1-methyl-3-octylimidazolium hexafluorophosphate [C8MIM][PF6]. Several factors affecting the microextraction efficiency, such as the type and volume of extracting solvent, type and volume of disperser solvent, salt concentration, and pH of the sample solution have been optimized. The calibration plots were linear in the range of 0.1-20 mg L^-1 for carbamazepine and 0.3-40 mg L^-1 for lamotrigine with detection limits of 0.04 mg L^-1 for carbamazepine and 0.07 mg L^-1 for lamotrigine in plasma samples. The results confirm the suitability of the presented method as a sensitive method for the analysis of the target analytes in urine and plasma samples.