Micellar Liquid Chromatographic Method for Determination of Moxifloxacin and its Impurities

Abstract

A selective eco-friendly micellar HPLC method for investigation of moxifloxacin and related substances in presence of its degradation products, was developed. Central Composite Design was used to optimize the experimental conditions. The proposed method is based on isocratic elution on a C18 column, using 92.5% (v/v) biodegradable aqueous mobile phase containing 0.01 M sodium dihydrogenphosphate,0.15 M sodium dodecyl sulfate (SDS) and 0.5% triethylamine (v/v) with pH value set at 3.5 and 7.5% of propanol (v/v) as eco-friendly organic solvent. The flow rate and injection volume were 0.6 ml/min and 5 µl, respectively. Experiments were carried out at temperature 60 °C and detection was performed at 295 nm. The optimized method was validated.The method was found to be suitable for quantification of moxifloxacin and its related substances in moxifloxacin drug substance. The Green analytical procedure index (GAPI) demonstrated the superiority of the developed method.