Spectrophotometric Determination of 4-Acetamidophenyl N’-(Sulphanilamide) Acetate in Biological Fluids

Abstract

A simple, accurate and low cost spectrophotometric method is proposed for the determination of the synthesized paracetamol derivative; 4-acetamidophenyl N’-(sulphanilamide) acetate (APSA) in biological fluids. The spectrophotometric method is based on a condensation reaction between the alcoholic solution of APSA and acidic solution of p-dimethylaminobenzaldeyde to generate a yellow colored product. The linear range for the determination of APSA was 1-10 μg mL^-1 with molar absorptivity of 3.6877 × 10⁴ L mol^-1 cm^-1 and Sandell’s sensitivity of 0.001 μg cm^-2/0.001 absorbance unit. During the inter-day and intra-day analysis, the relative standard deviation for replicated determination of APSA was found to be less than 2% and accuracy was 99.2-101.6% and 99.1-101.3% in blood and urine samples, respectively. There was no interference from commonly used blood and urine sample. The developed spectrophotometric method was successfully applied to assess APSA in biological fluids.